Testing & Validation · Shelf-Life & Stability Testing

Shelf-life and stability testing, for the date on your pack.

Real-time and accelerated studies that track nutrients, oxidation, microbiology and texture — so your shelf life is set from data.

Guires Pet Nutrition Lab

Pet Food Shelf-Life & Stability Testing from Guires PNL

The date on a pet food pack is a claim that the product will still be safe, palatable and meet its label at the end of that period. Vitamins degrade, fats oxidise, moisture migrates and microbes grow at different rates in different formats.

We design shelf-life studies around the failure modes of your format, combining accelerated conditions for early answers with real-time storage for confirmation, and track the nutrients and quality markers that decide when a product is no longer fit to sell.

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What We Test

Shelf-Life & Stability Testing scope.

Each test is chosen for a reason — the stage of development, the format and the rules in your market.

Real-time studies

Storage at labelled conditions with scheduled pulls through the full shelf life.

Accelerated studies

Elevated temperature and humidity for an early indication of stability.

Nutrient stability

Vitamins, thiamine, taurine and functional actives tracked at each time point.

Oxidation

Peroxide value, anisidine value and rancidity markers for fat-rich products.

Microbiological stability

Moulds, yeasts and spoilage organisms in moist, soft and fresh formats.

Packaging studies

Barrier, moisture uptake and pack interaction across shelf life.

Scientific Challenges

What we solve for.

01

Thiamine losses

Thiamine keeps falling during storage, so end-of-life values decide overages.

02

Fat oxidation

Coated fats and omega-3s oxidise, affecting palatability and nutrition.

03

Accelerated vs real time

Accelerated conditions give early indications but must be confirmed in real time.

04

Format differences

Dry, wet, fresh and soft formats fail in different ways and need different markers.

How We Work

From question to evidence.

Define

Shelf-life target and failure markers.

Design

Conditions, time points and tests.

Store

Accelerated and real-time storage.

Pull & test

Scheduled testing at each time point.

Trend

Results analysed against limits.

Set

Shelf life, overages and storage advice.

Evidence

The science behind our approach.

Peer-reviewed findings that shape how we design and interpret this work.

Peer-reviewed evidenceShelf-Life & Stability Testing · 2 sources

Thiamine is lost in processing and storage

Canned cat food lost 17.8% of added thiamine over six months; thiamine deficiency outbreaks have been linked to low-thiamine cat food.
What we do

Thiamine measured in finished product and during storage, not only assumed from the formula.

  1. StudyDainton 2022, Front Vet Sci
  2. StudyChang 2017, J Feline Med Surg
Peer-reviewed evidenceShelf-Life & Stability Testing · 1 source

Retorting destroys thiamin

Retorting cut thiamin in canned cat food by 30.4%, whatever the container; time to reach target lethality ranged from 29.5 to 45.6 minutes by pack size.
What we do

Vitamins are measured after processing, and heat penetration is studied for each pack format.

  1. StudyDainton 2023, Front Vet Sci
Peer-reviewed evidenceShelf-Life & Stability Testing · 2 sources

Recontamination after the kill step

Salmonella stayed detectable on dry pet food for 19 months; E. faecium is a conservative surrogate for validating heat steps.
What we do

Kill steps are validated with surrogates where needed, and post-process hygiene is part of the transfer package.

  1. StudyLambertini 2016, Food Microbiol
  2. StudyBianchini 2014, J Food Prot
Deliverables

What you take away.

  • Stability protocol and schedule
  • Results at every time point
  • Trend analysis against limits
  • Recommended shelf life and storage conditions
  • Overage recommendations for sensitive nutrients

Ingredient science for shelf-life & stability testing

Every ingredient we choose for shelf-life & stability testing is assessed for nutrient contribution, digestibility, processing behaviour, palatability and the rules in your market — and sourced to your specification.

Ingredientsimages/pd-novel-proteins.jpg · 1600×1200
Insightsimages/article-7.jpg · 1600×1200

Market insights from Guires PNL scientists

Regulation updates, the latest research, recalls and market trends that shape shelf-life & stability testing — written by our formulation, testing and regulatory teams.

FAQs

Common questions.

How long does a shelf-life study take?

Accelerated studies typically run 3–6 months; real-time studies run for the full intended shelf life.

Can accelerated data alone set shelf life?

It gives an early indication, but we recommend confirming it with real-time data.

Do you include palatability?

Where needed, palatability can be checked at key time points with partner panels.

Client Testimonials · Testing & Validation

What our clients say.

Clients in the USA, UK and UAE on working with Guires PNL. Names are withheld at clients’ request.

“Their nutritional and stability testing produced documentation we could present to retailers and regulators. Having that validation in hand made our retail listing process far smoother.”
USAProduct Development ManagerUnited States
“As a small brand, independent validation of our nutritional claims was beyond our own resources. They verified our shelf life and nutrient retention and provided the data to back every claim.”
UKFounderUnited Kingdom
“They tested our formula under the heat and storage conditions of our region and delivered audit-ready reports. We launched knowing the product would perform exactly as specified.”
UAEQuality Assurance ManagerUnited Arab Emirates

Planning shelf-life & stability testing?

Tell us your product, your question and your market — our scientists will come back with a study design and next steps.

FEDIAFAAFCO

Every Guires PNL product is formulated to comply with the FEDIAF Nutritional Guidelines and the AAFCO Dog and Cat Food Nutrient Profiles. Neither body issues certificates to manufacturers, so compliance is verified independently against their published standards — and every product comes with a complete documentation package for regulatory submissions in the EU, US, Australia and Asian markets.

How we verify compliance →
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