Our Science · Pet Bioscience Lab

Pet Bioscience Lab — biology behind better nutrition.

Probiotics, fermentation, molecular testing and lab-based efficacy screening for pet food, treat and supplement brands — and for the ingredient companies that supply them.

4service areas
11bioscience services
5–100 Lfermentation scale
In vitroscreening before animal studies
Guires Pet Nutrition Lab

Pet Bioscience Services from Guires PNL

Many of the newest pet nutrition products depend on biology: live probiotics, fermented and enzyme-processed ingredients, bioactive peptides and functional actives. Proving these work — that strains survive, actives stay stable and ingredients do what is claimed — needs biological methods beyond routine nutrient testing.

The Pet Bioscience Lab brings fermentation, molecular biology, cell-based screening and protein analysis into one facility. It supports pet food, treat and supplement brands and ingredient companies with strain development, identity and count testing, in vitro efficacy screening and active-ingredient analysis before products move to feeding studies.

Start your project with Guires PNL
Services

What the bioscience lab does.

Choose a service area to see what we deliver, the equipment behind it and the functional benefits it supports.

Probiotics & Fermentation

From strain to stable powder — grown, concentrated and freeze-dried in-house.

01

Probiotic & postbiotic development

Strain screening, growth optimisation, concentration and freeze-drying — plus heat-treated postbiotics made from the same strains.

You receive
  • Strain screening report
  • Fermentation and drying protocol
  • Lab and pilot-scale powder batches
Equipment
Fermentors 5–100 LContinuous centrifugeTFF systemsLyophilizerBiosafety cabinet
02

Survival & delivery testing

Will your strain survive extrusion, drying, shelf life and the dog or cat gut? We test viability under simulated processing and digestion.

You receive
  • Viability counts across conditions
  • Coating and post-process addition advice
Equipment
IncubatorsWater bath shakerspH meterHot air oven
03

Fermented & enzyme-processed ingredients

Fermented functional ingredients, enzyme-hydrolysed proteins for sensitive-pet diets and palatant digests, developed at lab and pilot scale.

You receive
  • Process protocol
  • Pilot batches for trials
Equipment
FermentorsHomogenizerCentrifugesRotavaporLyophilizer
04

Freeze-dried prototypes

Lab-scale freeze-dried treats, toppers and probiotic powders for prototypes, sampling and early studies.

You receive
  • Prototype batches
  • Moisture and stability data
Equipment
LyophilizerDeep freezersSpeed vac

Molecular & Microbiology Testing

Knowing exactly which microbes are present — and how many.

01

Strain identity & live counts

Confirm the identity of probiotic strains and isolates, and count live cultures in finished products through shelf life — the evidence behind “contains live cultures” claims.

You receive
  • Identity report
  • Viable count over shelf life
Equipment
MALDI-TOF mass spectrometerGenetic analyzerReal-time PCRIncubators
02

Rapid pathogen screening

PCR-based screening for Salmonella, Listeria and STEC E. coli in raw, fresh and freeze-dried products and environmental samples.

You receive
  • Screening results
  • Confirmation route for positives
Equipment
Real-time PCRThermal cyclerBiosafety cabinetAutoclaves
03

Biomarkers & microbiome support

Blood biomarkers and immune markers from partner feeding studies, plus faecal DNA extraction and targeted PCR for specific gut bacteria.

You receive
  • Biomarker data sets
  • Targeted microbiome results
Equipment
Plate readerFlow cytometerReal-time PCR−80 °C freezersCryocans

Lab Efficacy & Digestibility Screening

Test ideas in the lab before they reach an animal study.

01

Cell-based efficacy screening

Screen actives in cell models for gut-barrier support, immune response, antioxidant activity and inflammation markers — so only the most promising go forward to feeding studies.

You receive
  • Screening report with dose–response
  • Shortlist for animal studies
Equipment
Cell culture facilityCO₂ incubatorFlow cytometerFluorescence microscopePlate reader
02

Simulated digestion

Model dog or cat digestion in the lab to compare protein digestibility and how much of an active is released from a recipe or ingredient.

You receive
  • Comparative digestibility results
  • Bioaccessibility of actives
Equipment
Shaking water bathsIncubatorspH meterCentrifugesUV-VisPlate reader

Protein & Active Analysis

Characterising the ingredients that make functional claims.

01

Protein, peptide & hydrolysate profiling

Check how far proteins are broken down in hydrolysed diets, profile collagen and other peptides, and compare protein profiles for novel proteins such as insect.

You receive
  • Molecular-weight profile
  • Protein pattern comparison
Equipment
1D and 2D electrophoresisGel documentationMALDI-TOF/TOFFPLC
02

Active-ingredient stability

Measure active levels and track their stability over shelf life — vitamins, taurine, NAD+ precursors and botanical markers — with accelerated storage and oxidation checks.

You receive
  • Stability data over time
  • Overage recommendation
Equipment
UPLCUV-VisPlate readerBOD incubatorsParticle size analyzer
Who It’s For

Built for the people making claims.

01

Supplement brands

Live-culture counts, active stability and evidence for functional claims.

02

Ingredient companies

Strain development, efficacy screening and characterisation data for new ingredients.

03

Pet food brands

Probiotic delivery, pathogen screening for raw and fresh lines, and digestibility comparisons.

04

Researchers & universities

Molecular, cell and protein work as part of joint studies.

Equipment

A complete biotech lab, under one roof.

Every instrument behind our bioscience services — 52 in all. Filter by lab area.

Fermentation & bioprocessing10

  • Fermentor – 5 LStrain screening and process development runs
  • Fermentor – 40 LScale-up and optimisation batches
  • Fermentor – 100 LPilot batches for trials and sampling
  • Lab TFF systemTangential-flow concentration at bench scale
  • Pilot TFF systemConcentration and clarification at pilot scale
  • Continuous high-throughput centrifugeBiomass harvest from fermentation broth
  • Homogenizer / cell disrupterCell lysis and emulsion preparation
  • LyophilizerFreeze-drying cultures, toppers and prototypes
  • RotavaporGentle solvent removal and extract concentration
  • Speed vacConcentrating small-volume samples

Molecular biology5

  • Real-time PCRStrain identity, live counts and pathogen screening
  • Thermal cyclerDNA amplification for identification work
  • Genetic analyzerFragment and sequence analysis
  • ElectroporatorStrain transformation for research work
  • Hybridization ovenProbe-based molecular assays

Protein & mass spectrometry8

  • MALDI-TOF/TOF mass spectrometerMicrobial identification and peptide profiling
  • 1D electrophoresisProtein size profiling
  • 2D electrophoresisHigh-resolution protein separation
  • Semi-dry transfer unitWestern blot transfer
  • Gel documentation – basicRoutine gel imaging
  • Gel documentation – advancedQuantitative gel and blot imaging
  • FPLC systemProtein purification
  • Chromatography systemFractionation of hydrolysates and actives

Cell biology & imaging7

  • Cell culture facilityCell-based efficacy screening
  • CO₂ incubatorControlled cell-culture growth
  • Flow cytometerImmune-cell and viability analysis
  • Fluorescence microscopeStained-cell and marker imaging
  • Inverted microscopeLive cell-culture observation
  • Biosafety cabinetSafe handling of cultures and cells
  • Laminar air flowSterile preparation work

Analytical7

  • UPLCActive-ingredient and stability assays
  • Plate readerHigh-throughput assays and ELISA
  • UV-Vis spectrophotometerConcentration and colour-based assays
  • Particle size analyzerParticle and droplet sizing
  • Cell counterFast, accurate cell counts
  • pH metersProcess and sample pH control
  • Analytical balancesPrecise weighing for every method

Separation, storage & support15

  • Ultra centrifugeHigh-speed separation of fine fractions
  • Large and medium centrifugesRoutine sample and biomass separation
  • Table-top centrifugesEveryday bench separation
  • Horizontal autoclaveSterilising media and equipment
  • Vertical autoclaveSterilising media and waste
  • −80 °C freezerLong-term culture and sample storage
  • −20 °C freezerReagent and sample storage
  • CryocansLiquid-nitrogen culture banking
  • Cold roomControlled storage for materials and samples
  • BOD incubatorTemperature-controlled incubation and shelf-life work
  • Shaker incubatorAerated culture growth
  • Water-bath incubatorSimulated digestion and temperature-held assays
  • SonicatorCell disruption and sample dispersion
  • OvensDrying and moisture work
  • Water purification systemLab-grade water for every method
Quality & complianceGMP-certified partner facility for production-scale batchesFSSAI-licensed and ISO 9001:2015-certified operationsBiosafety cabinets and controlled cold storage for cultures
How We Work

From question to evidence.

Define the question

What you need to prove, for which species, product and market.

Design the study

Methods, controls, sample numbers and endpoints agreed up front.

Run in the lab

Fermentation, cell, molecular or protein work in our bioscience lab.

Analyse & interpret

Statistics and a scientist’s interpretation against your goal.

Report

A clear report you can use for development decisions and claim files.

Next step

Scale-up, a feeding study with partner panels, or formulation.

Scope

What we do here — and what partners do.

The bioscience lab complements our wider testing network. Routine nutritional and contaminant analysis stays with Guires Analytical Lab and qualified partner laboratories.

  • In-house: fermentation, freeze-drying, cell-based screening, molecular testing, protein and peptide profiling, active stability
  • Through partners: proximate, mineral, fatty-acid, mycotoxin and heavy-metal analysis
  • Through partners: full microbiome sequencing — we prepare samples and run targeted PCR
  • Feeding and palatability studies run with partner expert panels
  • GMP: production-scale batches run at our GMP-certified partner facility
Nutrition ScienceSpecies, life stages and functional nutrition. Explore →
Testing & ValidationNutritional, safety and stability testing. Explore →
Contract ResearchStudies, reviews and dossiers. Explore →
Ingredient LibraryThe ingredients behind the label. Explore →
Insights

Guires PNL quality, compliance and safety

Every project at Guires Pet Nutrition Lab runs inside an ISO 9001:2015 certified quality system and FSSAI-licensed operations, with documented methods, calibrated instruments, traceable raw materials and reviewed reports — so the results behind your product can be checked and defended.

Quality

Have a strain, an active or a claim to test?

Tell us what you need to prove — we will design the bioscience work around it.

FEDIAFAAFCO

Every Guires PNL product is formulated to comply with the FEDIAF Nutritional Guidelines and the AAFCO Dog and Cat Food Nutrient Profiles. Neither body issues certificates to manufacturers, so compliance is verified independently against their published standards — and every product comes with a complete documentation package for regulatory submissions in the EU, US, Australia and Asian markets.

How we verify compliance →
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